| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nelson, Clara A (10)
|
VAPSDN |
41.93 |
39.40 |
-2.53 |
| 2 |
Pew, Isabella F (10)
|
VAPSDN |
43.00 |
40.25 |
-2.75 |
| 3 |
Liebler, Emma M (10)
|
VA757 |
45.07 |
40.85 |
-4.22 |
| 4 |
Fary, Brenna E (10)
|
VAECAT |
42.28 |
42.35 |
0.07 |
| 5 |
Palm, Ryan M (10)
|
VACGBD |
|
44.70 |
- |
| 6 |
Robertson, Logan A (10)
|
VAWAC |
|
45.20 |
- |
| 7 |
Aquino, Meagan E (10)
|
VAECAT |
50.75 |
45.33 |
-5.42 |
| 8 |
Cleary, Peyton M (10)
|
VA757 |
|
45.91 |
- |
| 9 |
Campbell, Amelia D (10)
|
VASEVA |
40.12 |
46.37 |
6.25 |
| 10 |
Jimenez, Saniya N (10)
|
VASEVA |
49.01 |
47.09 |
-1.92 |
| 11 |
McGowan, Elly G (10)
|
VASEVA |
47.15 |
47.32 |
0.17 |
| 12 |
Stupar, Aliyah G (10)
|
VASEVA |
48.99 |
47.79 |
-1.20 |
| 13 |
Shaltayeva, Victoria (10)
|
VASEVA |
50.29 |
48.52 |
-1.77 |
| 14 |
Daniel, Campbell S (10)
|
VA757 |
|
48.53 |
- |
| 15 |
Wojcik, Melissa A (10)
|
VASEVA |
48.68 |
48.86 |
0.18 |
| 16 |
Segura, Mara J (10)
|
VATSU |
54.00 |
50.10 |
-3.90 |
| 17 |
Rodrigues, Gabriella A (10)
|
VASEVA |
54.27 |
51.17 |
-3.10 |
| 18 |
Banks, Hannah M (10)
|
VASEVA |
55.29 |
51.18 |
-4.11 |
| 19 |
Veith, Dori A (10)
|
VA757 |
|
52.85 |
- |
| 20 |
Townsend, Leah C (10)
|
VAWAC |
54.83 |
54.50 |
-0.33 |
| 21 |
Tomblin, Leah S (10)
|
VACGBD |
55.68 |
56.03 |
0.35 |
|
Hernandez, Camilla E (10)
|
VACGBD |
|
NS |
- |